This game has been posted before but here is an update and done as a static build (uclibc/tinyX11) which should enable it to work on most past and future releases of puppy...
I have removed the build-in GTK2-launcher as the original game menu take care of most of the things anyway.
* eduke 32 sitelink
* Download DUKE3D.GRP datafile [NB: by downloading this file you agree that you have a valid license for dukenukem3d yourself...link to shareware version here which need no license] - put it in same directory as eduke32 or in your /root/.eduke32 directory.
* Download static build of eduke32 (eduke32_20120816-2915)